A New York doctor who returned from treating patients in Guinea this month has become the city's first person to test postive for ebola.
Craig Spencer, 33, has been held at an isolation ward at Manhattan's Bellevue Hospital, a designated centre for dealing with the virus.
His apartment in Harlem has been sealed off and authorities are trying to track down those who came into contact with him in the days before he was admitted to hospital.
The doctor, originally from Detroit, Michigan, had been working in west Africa for medical charity Doctors Without Borders.
Dr Spencer arrived in New York from Guinea on 17 October and reported coming down with a fever and diarrhoea on Thursday.
Before he was admitted to hospital, he travelled on the subway, went to a bowling alley in Brooklyn and then took a cab back to Manhattan.
New York Mayor Bill de Blasio said there was no reason for people to worry.
He told a news conference: "Ebola is an extremely hard disease to contract. It is transmitted only through direct contact with infected persons' blood or other bodily fluids.
"Travelling on the same subway car ... does not ... put someone at risk."
Two of Dr Spencer's friends and his fiancee have been placed in quarantine. The fiancee is in hospital, but all three are healthy.
A fourth person, a taxi driver, did not come into close contact with him.
Guinea is one of the three worst-hit countries in the latest ebola outbreak, along with Sierra Leone and Liberia. Nearly 4,900 people have died so far.
Mali had earlier become the latest country to admit one of its residents had tested positive for the disease.
A health ministry official said the girl had just arrived from Guinea, where her mother died of the virus a few weeks before.
Bellevue is one of three Manhattan hospitals and eight in the state of New York equipped to handle patients diagnosed with ebola.
The ongoing threat from ebola was underlined on Thursday by the findings of a study in medical journal Lancet Infectious Diseases, which estimated that by 15 December, part of Liberia could suffer 171,000 cases of the disease.
The total number of cases would affect one in eight of Montserrado county's 1.38 million population, with at least 90,000 expected to die.
The study said that number could be cut substantially if quarantine procedures are put in place and standard medical techniques - like using soap, bleach, masks and gloves extensively - are put in place.
In the UK, David Cameron pledged a further £80m for the fight against the disease.
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